Glucose concentration on fibroblast proliferation

Objective To find the relationship between glucose concentration and the proliferation of fibroblast.Methods The proliferation of mouse fibroblast was tested in different concentration of glucose at 12h,24h,48h with CCK kit.The amount of the out-cell type I collagen fibers was tested by the mouse type I collagen Elisa kit,and the expression of α-smooth muscle actin(α-SMA) tested by immunochemistry.Results We found that under the medium of low concentration,the proliferation was much more than that in the high concentration groups after 12h,24h and 48h(P0.01),namely the concentration was negatively correlated with the proliferation.We tested the out-cell type I collagen fibers using the mouse type I collagen Elisa kit,but there was no difference between each group(P0.05).About the expression of mouse α-SMA,positive correlation was found between the glucose concentration and the expression of the mouse α-SMA.Conclusion At the original augmentation of the blood glucose,the proliferation of fibroblast is not different.But when it gets over a level,the proliferation will be depressed,which can lead to the delay of the healing of raw surface;the concentration of glucose has nothing to do with the out-cell type I collagen fibers;the concentration of glucose is a factor which enhances the variation from fibroblast to myofibroblast,and the concentration of glucose is positively correlated with the tendency of the variation.