The influence of serum medium upon the levels of type I collagen and type III collagen and their ratio of the in vitro cultured L929 cell line of the mouse fibroblast

Objective To explore the influence of different blood serum concentration and different in vitro culture time upon the levels of type Ⅰ and Ⅲ collagen and their ratio of L929 cell line of the mouse fibroblast.Methods L929 cell line of the mouse fibroblast was cultured in vitro,and ABC-ELISA was used to measure the concentrations of type Ⅰ and Ⅲ collagen and their ratio.Results Except 0% serum concentration group which type Ⅰ and Ⅲ collagen displayed no significant changes but only a slightly increasing tendency,type Ⅰ and Ⅲ collagen in other serum concentration groups displayed an increasing state and increased most obviously from the 6th to 8th day.The ratio of type Ⅰ to Ⅲ changed differently in different concentrations of blood serum for different growth time.In 20% and 40% serum concentration groups,the ratio of type Ⅰ to Ⅲ increased gradually with the cell growth time.In other serum concentration groups,the ratio of type Ⅰ to Ⅲ did not increase obviously with the cell growth time.Conclusions Different blood serum concentration and different in vitro culture time of L929 cell have a certain influence upon the type Ⅰ and Ⅲcollagen and their ratio.This provides experimental data for future improvement of in vitro cell culture environment.