Improvement of the Culture Medium of Human Dermal Papilla Cells

Objective:To improve the culture medium of human dermal papilla cells so that the primary culture of dermal papilla cells could be established promptly and effectively.Methods:The dermal papilla cells were cultured in Dulbecco's modified eagle medium(DMEM) containing 100 ml/L FCS and dermal papilla cell medium(DPCM)respectively.The biological characteristics and morphological behavior of the above cells were compared in vitro.Results:The dermal papilla cells could grow better in the dermal papilla cell medium than in the Dulbecco's modified eagle medium(DMEM)containing 100 ml/L FCS and the former could be subcultured to 18-20 passages.Conclusion:Dermal papilla cell medium stimulates the growth of dermal papilla cells more remarkably than those of the Dulbecco's modified eagle medium(DMEM)containing 100 ml/L FCS.We think of the dermal papilla cell medium as a better culture medium of human dermal papilla cells.