Selection of reference genes for transcription expression analysis in the mouse

Objective To evaluate the sellection of suitable internal reference genes for normalization of gene expressions in mouse.Methods The expression levels of six housekeeping genes including GAPDH,HPRT1,B2M,PPIA,ACTB and 18S rRNA in the hypothalamus,pituitary and ovarian tissues and two developmental stages of C57BL/6J and C3H/HeJ mouse strains were assessed by quantitative real-time PCR(qPCR).Results The variation of expression levels was analyzed using geNorm program and demonstrated that GAPDH and HPRT1 were the most stable genes in different tissues at various developmental stages.PPIA,18S rRNA and ACTB were less stable,whereas B2M expression was almost undetectable in different tissues during development.Conclusions The successful selection of these two most stable housekeeping genes GAPDH and HPRT1 proves its necessity and feasibility,and provide the methodology for housekeeping gene selection as well.