Selection of Control Genes in Transcription Analysis of Gene Expression

At present, transcription analysis of gene expression commonly uses a single housekeeping gene as control for normalization. Through quantitative real-time PCR expression the levels of 6 housekeeping genes(including RPL13A,UBC,EIF4A,B2M,GAPDH,and ACTB) in BEL-7402 cell line were estimated under the role of a new tripeptide compound-YSV. Differences in expression levels were observed by analysis of geNorm program,and RPL13A, UBC were finally determined as suitable internal control genes. In conclusion, the necessity of choosing control genes was proved, and a good way was introduced to select control genes when experiments were handled by different empirical factors(especially under the effect of new materials).