The Content of Total Phenolic Acid in Evodia Rutaecarpa(Juss.)Benth and Its Processed Product

Objective:To establish a method for determining the contents of total phenolic acid in Evodia rutaecarpa(Juss.)Benth and its processed product.Methods:Total phenolic acid was determined by the method of spectrophotometry at 760nm with chlorogenic acid as reference material.In the systematic solution,0.3% dodecyl sulfonic acisodium salt 2mL,and mixed solution with 1% K3\[Fe(CN)6\]-1% were added.And also FeCl3(1∶1)2mL.with 0.1mol/L hydrochloric acid was added to the solution diluted to scale.Results:The contents of total phenolic acid were 29.89% and 30.44%.in the two kinds of Evodia rutaecarpa(Juss.)Benth,there was no notable difference by t test(P0.05).The contents of total phenolic acid was the highest in its processed product by vinegar, the second was liquorice, the others were notable lower than Evodia ru- taecarpa(Juss.)Benth,there was notable difference by t test(P0.01).Conclusion:The contents of total phenolic acid were different in its different processed product.The method was simple,accurate and rapid.It was a better method to determine the contents of total phenolic acid in Evodia rutaecarpa(Juss.)Benth.