The Blocking of Dengue Virus Replication by the Recombinant Alphavirus Containing Dengue-2 PrM Gene

Based on the resistance of the recombinant alphavirus containing dengue-2 PrM gene against dengue virus, replications of three other dengue serotype viruses were blocked by this recombinant virus. The recombinant plasmid (pSF-rM 2) DNA containing sense- or antisense-PrM gene and helper vector DNA were transcribed into RNAs in vitro, respectively. These transcribed RNAs were cotransfected into the culture of BHK cells by electroporation and were packaged into the recombinant virus particles. After these recombinant viruses were activated, BHK cells were infected by them and challenged with other three type dengue viruses. The blocking of dengue virus replication was observed by immunofluorescence. The results show that the recombinant deugue-2 PrM alphavirus can also inhibit replications of types 1,3 and 4, and the resistance of replications of type 1 and 4 were higher than that of type 3 virus. The recombinant antisense-PrM alphavirus can completely block replications of dengue type 1,3 and 4 viruses. But the recombinant sense-PrM alphavirus can not completely block replication of type 3 virus, when the transfected host cells were challenged with dengue viruses at 10 3 TCID 50 . These results might lay a foundation for exploring a new way to cure the dengue disease.