Preparation and Biological Identification of Recombinant SFV Virus Containing the PrM-E Gene of Chinese Dengue 2 Virus

To construct the recombinant SFV containing the PrM E gene, the SFV expression vector DNA, which contained the PrM E gene of Chinese dengue 2 virus, and the helper vector DNA were linearized and transcribed in vitro , respectively. Then the 2 RNA transcripts were directly coelectroporated into BHK cells. The cell supernatants were demonstrated to contain recombinant SFV virus with RT PCR. After being activated with α chymotrypsin, the recombinant virus could make BHK cells produce CPE. Furthermore, the proteins specific to dengue 2 virus could be detected in the infected cells by indirect immunofluorescence technique. The results laid a foundation for the study of the immunogenicity of the recombinant SFV virus and provided a new way to develop vaccines for dengue virus.