2010Mailei zuowu xuebaoRequires access

Genetic Diversity and Genetic Relationships among of Barley Genotypes Using SSR Markers

Zhao Chun-yan, Zeng YaWen, Pu XiaoYing

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Abstract

The genetic diversity and genetic relationships of 42 barley varieties(lines) were analyzed by 42 pairs of SSR makers.The results showed that the 42 pairs of SSR primers separated on the 7 chromosomes of barley,detected polymorphism at 112 alleles in total.The average number of alleles per SSR locus was 2.591 with a range from 2 to 5.The value of allelic polymorphism formation content (PIC) ranged from 0.321 to 0.857 with an average of 0.433 per SSR maker.UPGMA cluster analysis showed that the range of genetic similarity coefficient located between 0.321~0.857.At the similar-itg coefficient of 0.550,these barley varietiesC lines) could be classified into 4 groups.The majority of two-rowed beer and poly-rowed feed varieties(lines) were separately located in different groups,indicated that the genetic basis was rather extensive;In the same groups,the barley varieties(lines) had relatively narrow range,indicated that their genetic basis was more narrow;Relatively genetic background of barley landraces were complex.

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The genetic diversity and genetic relationships of 42 barley varieties(lines) were analyzed by 42 pairs of SSR makers.The results showed that the 42 pairs of SSR primers separated on the 7 chromosomes of barley,detected polymorphism at 112 alleles in total.The average number of alleles per SSR locus was 2.591 with a range from 2 to 5.The value of allelic polymorphism formation content (PIC) ranged from 0.321 to 0.857 with an average of 0.433 per SSR maker.UPGMA cluster analysis showed that the range of genetic similarity coefficient located between 0.321~0.857.At the similar-itg coefficient of 0.550,these barley varietiesC lines) could be classified into 4 groups.The majority of two-rowed beer and poly-rowed feed varieties(lines) were separately located in different groups,indicated that the genetic basis was rather extensive;In the same groups,the barley varieties(lines) had relatively narrow range,indicated that their genetic basis was more narrow;Relatively genetic background of barley landraces were complex.

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Available abstract

The genetic diversity and genetic relationships of 42 barley varieties(lines) were analyzed by 42 pairs of SSR makers.The results showed that the 42 pairs of SSR primers separated on the 7 chromosomes of barley,detected polymorphism at 112 alleles in total.The average number of alleles per SSR locus was 2.591 with a range from 2 to 5.The value of allelic polymorphism formation content (PIC) ranged from 0.321 to 0.857 with an average of 0.433 per SSR maker.UPGMA cluster analysis showed that the range of genetic similarity coefficient located between 0.321~0.857.At the similar-itg coefficient of 0.550,these barley varietiesC lines) could be classified into 4 groups.The majority of two-rowed beer and poly-rowed feed varieties(lines) were separately located in different groups,indicated that the genetic basis was rather extensive;In the same groups,the barley varieties(lines) had relatively narrow range,indicated that their genetic basis was more narrow;Relatively genetic background of barley landraces were complex.

Key concepts: UPGMA, Genetic diversity, Biology, Allele, Locus (genetics), Genetic similarity, Genetic relationship, Microsatellite

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