Genetic Diversity Analysis of Barley Varieties by SSR

The genetic diversity among 96 barley varieties(lines) from Different sources were analyzed by 107 pairs of SSR markers of good Polymorphism to provide genetic information in barley varieties.107 pairs of SSR primers with polymorphism were detected and 470 loci were measured in total.Each primer ccould detect 2～10 loci with 4.4 loci in average.Among those,319 loci have polymorphism which accounted 67.8 %.The polymorphism information content among the primers got the highest PIC of 0.75 and the lowest of 0.04,with the average of 0.42 among the 107 pairs.Clustering analysis showed that the genetic similarity coefficient(GS) ranged between 0.5480～0.9195.At the genetic similarity coefficient level of 0.674,these varieties could be clustered into 4 groups.Among them,42 of the winter barley varieties(lines) from different areas in China and introduced from Japan,and another 2 varieties(lines) introduced from USA were clustered into the same sub-class.45 varieties(lines) introduced from USA and 2 Chinese varieties(lines) were clustered into the same sub-class.That is to say,SSR genetic differences were mainly concerned with the material sources,but also a few materials appeared crossover classification in this study.There was problem that the genetic basis of domestic and introduced barley materials was narrow.The introduction of foreign barley varieties and their utilization needed to strengthen in Chinese barley breeding.