Effect of Tanshinone IIA on Proliferation of Vascular Smooth Muscle Cell Induced by Angiotensin II

Objective:To study the effects of tanshinone ⅡA(TSN) on proliferation of vascular smooth muscle cells(VSMCs) induced by angiotensinⅡ(AngⅡ).Methods:A cells proliferating model of VSMCs induced by AngⅡ was established.MTT colorimetric assay was used to measure the proliferation of VSMCs.The effects of TSN on CaN were detected by enzyme reaction phosphorus measurement,and the effects of TSN on the c-fos and c-myc gene expression examined by immunocytochemical staining and image analysis technique(A value).Results:The rat aortic SMCs were cultured in vitro successfully.CaN activities,cell proliferation activity and the expression levels of c-fos and c-myc were increased significantly in VSMCs induced by AngⅡ(P0.05,P0.01).However,the indexes above were obviously reduced in TSN-treated group as compared with Ang Ⅱ-treated group.Different concentrations of TSN(0.001,0.01,0.1 mg/L) decreased the CaN activity,cell proliferation activity,and the expression levels of c-fos and c-myc in a dose-dependent manner(P0.05 or 0.01).Conclusion:AngⅡ stimulated the proliferation of VSMCs significantly,which could be inhibited by TSN in a dose-dependent manner by reducing the CaN activity and the expression levels of c-fos and c-myc.