2006•Lishizhen Medicine and Materia Medica ResearchRequires access

Experimental Study on the Effects of TetramethyIpyrazine(TMP) on Calcineurin(CaN) and c-fos Gene Expression in the Preliferation of Vascular Smoo th Muscle Cells(VSMCs) Induced by AngII

Desheng Luo

Abstract

ObjectiveTo study the effects of tetramethylpyrazine(TMP)on calcineurln(CaN)and C-fos gene expression in theproliferation of vascular smooth muscle cells(VSUCs) treated by AngⅡ.MethodsA cell proliferating model of VSMCs induced by angiotensinlI(AnG Ⅱ) was established;the effect of tetramethylpyrazine(TMP) on calcineurin(CaN)was detected by enzyme reaction phosphorus measurement;the effect of etramethylpyrazine(TMP)on c-fos gene expression was observed by immunocytochemlcal stain and image analysis technology(A value).ResultsCell proliferation activity,CaN activity and the expression levels of PCNA increased significantly in VSMCs proliferation induced by Ang Ⅱ(P0.01).While treated with TMP,the index was obviously reduced compared with Angll group(P0.01).ConclusionThe VSMCs proliferation induced by AngⅡ can be inhibited by TMP significantly,and the inhibiting mechanism of TMP may be related to restraining the expression of c-fos in dose and time-dependent manner.

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Key concepts: Tetramethylpyrazine, Vascular smooth muscle, Calcineurin, Gene expression, c-Fos, Chemistry, Cell growth, Immediate early gene