Antioxidant Effect of Wheat Germ Bioactive Peptides in D-galactose-induced Aging Mice

Objective:To explore the antioxidant effect of wheat germ bioactive peptides (WGBP) in aging mice induced by D-galactose. Methods:Fifty male Kunming mice were randomly divided into five groups,control group,model group,and WGBP low-,medium-and high-dose groups. Except the control group,all the other groups were subjected to subcutaneous injection of D-galactose solution at a dose of 600 mg/(kg bw·d). Mice were treated with WGBP at doses of 200,800 mg/(kg bw·d) and 1000 mg/(kg bw·d) once a day for the creation of the WGBP low-,medium-and high-dose groups. The activities of SOD and GSH-Px,T-AOC and the content of MDA in serum,brain,liver and heart were determined after a trial period of 45 days. Results:The activities of GSH-Px,SOD and T-AOC exhibited a significant increase (P 0.01) in serum,and the activities of SOD and T-AOC also exhibited a significant increase in heart (P 0.01) due to WGBP treatment. The activity of GSH-Px in brain and liver exhibited an obvious increase (P 0.05); meanwhile,an obvious increase of SOD activity in brain was also observed (P 0.05). Furthermore,the content of MDA in serum and the above organs revealed a reducing trend (P 0.05). Conclusion:WGBP has good antioxidant effect in vivo as expected.