Expression and Significance of Twist2 and E-cadherin in Cervical Squamous Cell Carcinoma

Objective:Study Twist2 and E-cadherin expression in cervical intraepithelial neoplasia and cervical squamous cell carcinoma,and explore their roles and significance in the process of cervical cancer. Methods:Using western blot(Western blot) and immunohistochemistry method to detecte Twist2,E-cadherin expression in normal cervical tissue(control group),group CIN Ⅰ,CIN Ⅱ- Ⅲ group,cervical squamous cell carcinoma. Results:The differences of the positive expression rate of Twist2 and E-cadherin in four groups were statistically significant(χ2=48.163,P=0.000;χ2=43.169,P=0.000). The positive expression rate of Twist2,E-cadherin in cervical squamous cell differentiation,clinical stage,lymph node metastasis between the groups,the difference was statistically significant(P0.05),no statistically significant difference between the different age groups(P0.05). Twist2 and E-cadherin expression was negatively correlated(r=-0.401,P=0.003) in cervical squamous cell carcinoma. Control group,CIN group,cervical squamous cell carcinoma relative expression levels of Twist2,Ecadherin difference was statistically significant(F=652.225,P=0.000;F=299.112,P=0.000). Conclusions:Twist2,E-cadherin may participate in the process of malignant transformation of cervical squamous cell carcinoma,joint detection Twist2,E-cadherin help to determine the degree of malignancy of cerical cancer and its prognosis.