Expression of Twist and Bcl-2 in cervical squamous epithelium lesion tissue

Objective: To investigate the expression and significance of Twist protein and Bcl-2 protein in cervical squamous epithelial lesions.Methods: A total of 26 cases with normal cervical tissues,42 cases with cervical intraepithelial neoplasis Ⅰ(CINⅠ),74 cases with CINⅡ-Ⅲ,70 cases with cervical squamous cell carcinoma were collected,the expression of Twist and Bcl-2 protein in cervical tissues was detected by immunohistochemistry,and its relationship with clinical biological behaviors of cervical cancer was analyzed.Results:The expression rates of Twist protein in normal control group,CIN Ⅰgroup,CIN Ⅱ-Ⅲ group and cervical cancer group were 0%(0/26),26.2%(11/42),62.2%(46/74) and 72.9%(51/70),the expression rates of Bcl-2 protein were 0%(0/26),19.0%(8/42),43.2%(32/74) and 68.6%(48/70),respectively,the differences of four groups were statistically significant(P0.01).Among cases with cervical squamous cell carcinoma,Twist and Bcl-2 protein positive rate in poorly differentiated tumor group was higher than high,moderately differentiated tumor group,the positive rate of lymph node metastasis was higher than those without lymph node metastasis,the positive rate of ⅡB-ⅢA period was higher than Ⅰ-ⅡA period,the difference were all statistically significant(P0.05) while no significant difference was observed between the different age groups(P0.05).The high expression of Twist and Bcl-2 protein were positive correlated(P0.01).Conclusions: Twist and Bcl-2 protein expression increases gradually in the development of cervical squamous cell intraepithelial lesions,the two protein were positive correlated and could be used as valuable biological marker to detect cervical squamous intraepithelial early lesions and determine biological behavior of cervical squamous cell carcinoma.