GC determination of palmitic acid,oleic acid and linoleic acid in Litsea cubeba(Lour.)Pers.

Objective:To develop a method for the determination of palmitic acid,oleic acid and linoleic acid in Litsea cubeba(Lour.)Pers.Methods:The sample was determined with capillary column DB-17(30 m×0.25 mm, 0.25 μm)by GC.Temperature programs was 170℃ programmed to 250℃ at 5℃·min~(-1) for 10 min;the injector temperature was 250℃;the detector temperature was 250℃;the flow rate was 1.2 mL·min~(-1);split injection was conducted with split radio of 100:1;the column head pressure 98.3 kPa.Nitrogen was used as carrier gas.Results: Palmitic acid showed a good linear relationship in the range of 0.040-0.400 mg·mL~(-1)(r=0.9995)and the av- erage recoveries(n=3)were 98.3%-102.3%,RSD≤2.0%;oleic acid showed a good linear relationship in the range of 0.067-0.584 mg·mL~(-1)(r=0.9999)and the average recoveries(n=3)were 97.6%-102.2%,RSD ≤4.1%;linoleic acid showed a good linear relationship in the range of 0.099-0.869 mg·mL~(-1)(r=0.9997)and the average recoveries(n=3)were 95.7%-99.3%,RSD≤1.1%.Conclusion:The method is sensitive,simple and accurate for the determination of palmitic acid,oleic acid and linoleic acid in Litsea cubeba(Lour.)Pers.