Cloning of soluble hTRAIL tumor-specific vector and expression in nasopharyngeal carcinoma cell line CNE-2

Objective To clone human soluble TRAIL cDNA and construct its eukaryotic expression vector for establishing experimental foundation for targeted gene therapy of nasopharyngeal carcinoma(NPC).Methods The total RNA was extracted from human peripheral blood lymphocytes.TRAIL gene with interleukin 2 signal peptide was amplified by RT-PCR and cloned into the downstream of vector pGL3-181hTERT promoter to construct an eukaryotic vector,pGL3-181hTERT/TRAIL.The protein expressed in NPC cell line CNE-2 was identified by Western blot.Results Sequence of 613bp cDNA was obtained.Identified by enzyme digestion and sequence analysis,the recombinant eukaryotic expression vector for TRAIL gene was successfully constructed.The results of the Western blot showed that the TRAIL protein could be expressed in transfected CNE-2 cell.Conclusion Successful construction of eukaryotic expression vector pGL3-181hTERT/TRAIL provides the possibility for gene therapy of nasopharyngeal carcinoma.