Apoptotic effect of human maligant melanoma cell line A375 induced by tumor-specific recombinant TRAIL gene

Objective To explore the targeted gene therapy of tumors by using the TRAIL gene in combination with the telomerase promoter.Methods Total RNA was extracted from peripheral blood lymphocytes(PBL) whose proliferation had been stimulated.The TRAIL gene with an IL-2 signal peptide gene was amplified by RT-PCR and cloned into the vector pGL3-181hTERT downstream of the hTERT promoter to form a eukaryotic expressing vector.A375 cells were transfected by the recombinant vector and apoptosis of the transfected cells was evaluated by rypan-blue exclusion and transmission electron microscopy(TEM).Results We successfully constructed a recombinant eukaryotic expression vector for the TRAIL gene.The expressed product significantly induced the apoptosis of the A375 cells.Conclusion The recombinant eukaryotic expression vector pGL3-IL-181hTERT/TRAIL was successfully constructed,which provides the possibility for gene therapy of tumors.