A study of effect of trichostatin A on the expression of cell cycle related protein in K562 cell line

Objective To explore the effect of Trichostatin A(TSA) on cell proliferation and cell cycle in K562 cell lines.Method K562 cells were treated with(50-400)nmol/L Trichostatin A for 12-60 h and the growth inhibition rate of K562 cells was measured by MTT and cell apoptosis was inspected by flow cytometry(FCM).The expressions of cyclin E and retinoblastoma protein(Rb)in K562 cells was observed by immunohistochemistry at varied concentrations of TSA(100-300 nmol/L)for 24 hours.Result Proliferation of K562 cells was selectively inhibited by TSA in a dose-and time-dependent manner,with inhibitory rates of 37.83%-78.57%(P0.01)and K562 cell apoptosis rates were 14.38%-61.18% by FCM.Optical absorbance by immunochemistry for cyclin E was 0.215 4 ± 0.201 5 , 0.150 1±0.197 8 and 0.118 3±0.502 1 and that for Rb was 0.301 7±0.135 6 , 0.213 4±0.128 3 and 0.152 7±0.021 6 at varied concentrations of TSA(100-300 nmol/L),respectively,as compared to that of 0.327 4±0.109 3 and 0.418 6±0.187 0,respectively,in controls(P0.05),showing an decreasing trend with increase in TSA concentration in a dose-and time-dependent manner.Conclusion TSA could disturb the progress of cell cycle of K562 cells,with mechanisms of induction of cell apoptosis,regulation of G1/S checkpoints and down-regulation of the expression of cyclin E,leading to inhibition of the expression of Rb.