Simultaneous determination of quercetin,luteolin and kaempferol in Celosia cristata L. By RP-HPLC

Objective To develop an RP-HPLC for determination of three flavonoids(quercetin,kaempferol and luteolin)in Celosia cristata L.Method The HPLC column was ZORBAX SB-C18(150 mm×4.6 mm,5 μm),temperature was 30 ℃;detection wavelength was 360 nm ;mobile phase was menthol-0.2%phosphonic acid(45∶55),flow rate was 1.0 mL·min-1.Results Quercetin,luteolin and kaempferol regression equations were Y=1 504.412X+9.975 6,Y=1 991.745X+8.6051 and Y=567.591X+2.539 7,respectively ;Linear ranges were 5.5×10-2～19.3×10-2 mg·L-1,4.6×10-2～16.1×10-2 mg·L-1 and 12.6×10-2～43.9×10-2 mg·L-1 respectively ;corelation coefficents were between 0.999 92 and 0.999 98;recoveries were 94.68%,91.03% and 103.08%,respectively ;RSD were 0.35%,1.01% and 0.55%,respectively.Sample contents of quercetin,luteolin and kaempferol were 0.176,0.262 and 0.105 mg·g-1,respectively.Conclusions This method was convenient and effective,and suitable for the determination of quercetin,kaempferol and luteolin in Celosia cristata L.