RP-HPLC Determination of Quercetin and Kaempferol in the Leaves of Phyllanthus emblica L

Objective:To establish a quantification method for quercetin and kaempferol in the leaves of Phyllanthus emblica by HPLC.Method:HPLC is applied on the determination of quercetin and kaempferol in the leaves of P.emblica with C18 column(4.6 mm×250 mm,5 μm) and a mixture of acetonitrile-0.1% phosphoric acid (27:73) as a mobile phase with the flow rate at 1.0mL·min-1.Quercetin and kaempferol were detected at 360nm,column temperature was set at 30 ℃ .Result:Quercetin and kaempferol were linear in the range of 0.06～1.2 μg and 0.021～0.43 μg.The average recovery of quercetin and kaempferol was 98.83% with RSD 2.73% and 101.58% with RSD 2.21% ( n=6 ) respectively.Conclusion:The method can be applied to the quality control of the leaves of P.emblica L.