2005•Zhonghua zhengxing shaoshang waike zazhiRequires access
The influence of adrenaline on the expression of TGF-β_1, bFGF and I procollagen for hypertrophic scar fibroblasts in vitro
Chengde Zhang
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Abstract
ObjectiveTo investigate the influence of adrenaline on the expresson of TGF-β_1, bFGF and I procollagen for human normal and hypertrophic scar dermal fibroblasts cultured in vitro. Methods Human normal and hypertrophic scar dermal fibroblasts were propagated in a serum-free in vitro model with adrenaline for 24 hours. The human mRNA levels of bFGF, TGF-β_1 and I procollagen in fibroblasts were determined by RT-PCR. Levels of bFGF and TGF-β_1 in the supernatants of fibroblasts cultured in vitro were determined by enzyme-linked immunosorbent assay (ELISA). Results In our study, adrenaline caused statistically significant increase in the peak levels of bFGF for normal and hypertrophic scar fibroblast cell lines (P0.01). It also caused statistically significant decrease in the level of TGF-β_1 for normal and hypertrophic scar fibroblast cell lines. Modulation of normal fibroblasts with 0.05, 0.10 and 0.20 μmol/L adrenaline resulted in a statictically significant (P0.01) decrease in the expression of I procollagen mRNA. However, only 0.20 μmol/L adrenaline can decreased the mRNA expression of I procollagen in the hypertrophic scar fibroblasts. ConclusionsWe conclude from these results that adrenaline can increase the production of bFGF and decrease production of TGF-β_1 and I procollagen in human normal dermal and hypertrophic scar fibroblasts cultured in vitro.