The study of IL-10 gene polymorphisms and contents relationship between Han and Uygur in Xinjiang

Objective: To investigate the IL-10 gene promoter-1082G/A( rs1800896),-819C/T( rs1800871),-592C/A( rs1800872) polymorphism in difference between Han and Uygur,while gene polymorphism relationship with IL-10 contents. Methods: Using polymerase chain reaction and direct gene sequencing compare 124 cases of Han and 131 cases of Uighur healthy people the differences in genotype,allele frequency and haplotype frequencies. Two ethnic populations of serum IL-10 contents were measured by ELISA. Results: The AA,AG,GG frequency of IL-10-1082 loci: Han( 86. 29%,12. 9%,0. 81%),Uygur( 53. 44%,41. 98%,4. 58%) respectively; the CC,CT,TT frequency of IL-10-819 loci: Han( 7. 26%,50. 81%,41. 93%),Uygur( 24. 43%,51. 91%,23. 66%) respectively; the CC,CA,AA frequency of IL-10-592 loci: Han( 7. 26%,50. 81%,41. 93%),Uygur( 24. 43%,51. 91%,23. 66%) respectively; Han population have found nine genotypes and five haplotype which was ACC,ATA,GCC,ACA,ATC,and Uygur have found six genotypes and ACC,ATA,GCC haplotype; GCC was high-producer-type,ATA was low-producertype,ACC was mid-producer-type. Conclusion: The level of IL-10 in serum was significant difference in Han and Uygur,it regulated by genetics. IL-10 gene in loci of-1082G / A,-819C / T,-592C / A had significant differences in genotypes and alleles frequency in Han and Uygur,the distribution of genotypes has its own characteristics in Xinjiang Uygur population,this difference may be the one of genetic factors which affect the incidence and prognostic between different races.