Establishment of rat model of immune tolerance through intranasal treatment with ovalbumin

[Objective] To establish the rat model of immuine tolerance through intranasal treatment with ovalbumin.[Methods] Rats in immuine tolerance group received OVA intranasally on each of 3 consecutive days,followed by sensitization and challenge.Asthma group received sensitization and challenge only.24 h after the last challenge,bronchoalveolar lavage fluid(BALF)was collected,and cytology study was conducted.The IL-4,IL-5 and IFN-γ BALF levels and the OVA-specific serum IgE level were determined by ELISA.The lung tissue was obtained and histologic analysis was done through HE.[Results] Total cells number,the percentage of eosinophils(Eos),lymphocytes and neutrophils in BALF,the IL-4 and IL-5 BALF levels and the OVA-specific serum IgE level of immuine tolerance group were lower than that of asthma group,while its BALF IFN-γ level was higher than that of asthma group.Compared with asthma group,peribronchiole inflammatory cells infiltration and mucin production of immuine tolerance group were alleviated.[Conclision] Intranasal administration with ovalbumin can establish rats model of immuine tolerance successfully.