Detection of the growth kinetics of duck enteritis virus vaccine strain in vaccinated SPF duck

The dynamic distribution of duck enteritis virus(DEV) vaccine strain in the brain,thymus,spleen,kidney,liver and bursa of Fabricius of the SPF duck vaccinated with DEV vaccine strain were detected.The specific primers(RTF and RTR) were designed according to the gene of DEV US6 in GenBank and,the standard curve with satisfaction of specificity,sensibility and repeatability was established using SYBR GreenⅠ real-time fluorescent quantitative PCR method.Copies of DEV DNA were quantified in the collected tissues by the standard curve.In result,the DEV DNA was detected in the brain,thymus,spleen,kidney,liver and bursa of Fabricius after immunization,and the copies of DEV DNA in the liver,brain and kidney were higher than in other examined organs from the first day to the third week after immunization.Since being vaccination,the copies of DEV DNA were unstable until the ninth week.By the end of this experiment,the copies of virus DNA had fall to 1×105 copies in these tissues except for the kidney and bursa of Fabricius.The results showed that DEV with pantropic mainly distributed in the brain,liver and kidney.