Analysis of ThinPrep liquid based cytology combined with human papillomavirus DNA test in diagnosis of cervical lesions from 1786 cases

Objective:To analyzed main types,age distribution,infection condition of cervical high-risk human papillomavirus (HR-HPV) and further explore the clinic value of HPV DNA test combined with liquid based cytology in diagnosis of cervical lesions.Methods:A total of 1786 women were tested for 16 types of HR-HPV by PCR and ThinPrep liquid based cytology test (TCT). Women with positive TCT and positive HR-HPV were referred for colposcopy and biopsy.Results:①A total of 376 women (21.1%) were proved to be infected by HR-HPV. 342 women (19.1%) were identified wittr positive cytology,of which were atypical squamous cells of undetermined significance (ASCUS) and low-grade intraepithelial lesions (LSIL) (73.1%).② Among the women with positive TCT and positive HR-HPV received colposcopy and biopsy,the higher the grade of TCT was and the more consistent with colposcopy and biopsy. ③The infection rate of HPV was higher in young women of actively sexual life among the normal crowd (P0.05). There was no significant difference among positive TCT,positive HR-HPV and both positive TCT and positive HR-HPV above the grade of LSIL. ④The rate of HPV16 was higher than the rate of HPV18 and HPV51 in Zunyi (P0.05).Conclusion:The HR-HPV infection in female genital tract is one of the major risk factors in cervical cancer and cervical intraepithelial neoplasm (CIN),it is suggested that the prevention and cure of uterine cervical cancer be emphasized on the prevention of HPV infection,the screening of HPV infection and the intimately monitoring of the crowd HPV infected. The detection of TCT combined with HPV DNA is the optimal approach for the diagnosis of cervical lesions,which is promising to be used in further large samples in this area.