Effect of Liuwei Dihuang Decoction on renal interstitial fibrosis in 5 /6 nephrectomized rat model

Objective To observe the protection of Liuwei Dihuang Decoction on renal interstitial fibrosis,and to investigate its effects on hepatocyte growth factor ( HGF) and its receptor ( c-Met) in 5 /6 nephrectomy rats. Methods SD rats were divided into sham operation group,model group and Liuwei Dihuang Decoction group,and adopted 5 /6 nephrectomy to copied chronic renal interstitial fibrosis. A week after operation,rats were administrated every day for 8 weeks. Pathological changes of the remnant kidney were observed by HE and Masson staining,Immunohistochemistry was used to observe the expression of HGF and c-Met protein. Results the model group and the Liuwei Dihuang Decoction group both appeared renal fibrosis,the model group was more obvious; Compared with the sham operation group,the expression of HGF manifested obvious promotion in the model group ( P 0. 05) ,the expression in the Liuwei Dihuang Decoction group raised too ( P 0. 01) ,compared with the model group,the expression in the Liuwei Dihuang Decoction group were more serious ( P 0. 05) ; Compared with the sham operation group,the expression of c-Met manifested obvious promotion in the model group and the Liuwei Dihuang Decoction group ( P 0. 01) ,compared with the model group,the expression in the Liuwei Dihuang Decoction group manifested more serious ( P 0. 01) . Conclusion Liuwei Dihuang Decoction may promote the expression of HGF and c-Met in 5 /6 nephrectomy rats and inhibit the development of renal interstitial fibrosis.