Effects of Liuwei Dihuang Decoction on Expression of HIF-1α and Related Factors in Chronic Renal Failure Rats

Objective To explore the expression of HIF-1α,PDGF-A and PDGF-B in chronic renal failure rats,and observe the effect of Liuwei Dihuang Decoction on its expression.Methods Chronic renal failure model was made by 5/6 nephrectomy.Rats were divided into sham operation group,model group and Liuwei Dihuang Decoction group.Twelve weeks after modeling,the indexes of renal function were detected,HE staining was used to observe the renal pathological changes,Masson staining was used to observe the renal tissue collagen deposition,immunohistochemical method and RT-PCR method were used to detect the expression of HIF-1α,PDGF-A and PDGF-B in kidney tissue.Results The 24 h urine protein,blood urea nitrogen and creatinine were higher in model group and lower in Liuwei Dihuang Decoction group(P 0.01).HE and Masson staining showed renal interstitial fibrosis significantly and large amounts of collagen fibers deposition in model group,while renal interstitial fibrosis was reduced significantly and a small amount of collagen fibers deposition in Liuwei Dihuang Decoction group.Compared with the sham operation group,HIF-1α,PDGF-A and PDGF-B protein level and gene expression were significantly increased in model group(P 0.01).Compared with the model group,HIF-1α,PDGF-A and PDGF-B protein level and gene expression decreased significantly in Liuwei Dihuang Decoction group(P 0.01).The expression of PDGF-A and PDGF-B were positively correlated with HIF-1α(P 0.01).Conclusion HIF-1α may promote renal interstitial fibrosis through the up-regulation of PDGF-A and PDGF-B expression,Liuwei Dihuang Decoction can down-regulate the expression of HIF-1α,thereby inhibit renal interstitial fibrosis.