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2003•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Ginsenoside Rg1 and Ginsenoside Re in Hongshen

Deng Kai-ying

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Abstract

Objective:To improve on the HPLC method for determination of Ginsenosid Rg1 and Ginsenoside Re in Hongshen (Red Ginsen).Method:A C 18 column was used.The mobile phase consisted of CH-3CN-H-2O(20∶80) and the detection wavelength was 203nm.Results:The calibration curves were separately linear of Ginsenoside Rg1 and Ginsenoside Re between 0^6008～6^0080μg,0^4032～4.0320 μg;the average recoveries of Ginsenoside Rg1 and Ginsenoside Re were separately 98^5%(RSD1^3%,n6) and 99^2%(RSD1^1%,n6).Conclusion:This method is simple,quick,reproducible and is suitable for the quality control of Hongshen.

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What this paper is about

Objective:To improve on the HPLC method for determination of Ginsenosid Rg1 and Ginsenoside Re in Hongshen (Red Ginsen).Method:A C 18 column was used.The mobile phase consisted of CH-3CN-H-2O(20∶80) and the detection wavelength was 203nm.Results:The calibration curves were separately linear of Ginsenoside Rg1 and Ginsenoside Re between 0^6008～6^0080μg,0^4032～4.0320 μg;the average recoveries of Ginsenoside Rg1 and Ginsenoside Re were separately 98^5%(RSD1^3%,n6) and 99^2%(RSD1^1%,n6).Conclusion:This method is simple,quick,reproducible and is suitable for the quality control of Hongshen.

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Available abstract

Objective:To improve on the HPLC method for determination of Ginsenosid Rg1 and Ginsenoside Re in Hongshen (Red Ginsen).Method:A C 18 column was used.The mobile phase consisted of CH-3CN-H-2O(20∶80) and the detection wavelength was 203nm.Results:The calibration curves were separately linear of Ginsenoside Rg1 and Ginsenoside Re between 0^6008～6^0080μg,0^4032～4.0320 μg;the average recoveries of Ginsenoside Rg1 and Ginsenoside Re were separately 98^5%(RSD1^3%,n6) and 99^2%(RSD1^1%,n6).Conclusion:This method is simple,quick,reproducible and is suitable for the quality control of Hongshen.

Key concepts: Ginsenoside Rg1, Ginsenoside, Chromatography, Calibration curve, Chemistry, High-performance liquid chromatography, Ginseng, Medicine

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