Establishment of SSR fingerprint map and analysis of genetic similarity among 35 varieties in japonica rice(Oryza sativa L.)

The genomic DNA of 35 japonica rice varieties were amplified by 68 pairs of SSR primers,Forty-six primers could amplify stable polymorphism among the 35 varieties.6 varieties could be identified by single SSR marker,while the other 29 varieties could be identified by combination of different SSR fingerprint.All the 35 varieties could be distinguished each other by the fingerprint map constituted by 12 pairs of core SSR primers.The genetic similarity coefficients of the 35 varieties ranged from 0.27 to 0.98.Furthermore,all the varieties were classified into 4 groups,at the level of genetic similarity coefficient 0.82 according to cluster analysis by an un-weighted pair-group average method with arithmetic mean.As expected,the result of cluster reflected basically the genetic relatives based on the pedigree analysis.