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2006•Unpublished venueRequires access

Chromatographic Methods of Shaotangshang Gel

Wang Yan-ping

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Abstract

Objective: to establish the chromatographic methods of Shaotangshang gel for control the quality standard of Chinese medicine.Methods: TLC was performed to identify Rhizoma coptidis,Cortex phellodendri chinensis,Radix et rhizome rhei,Radix scutellariae.HPLC was used to determine content of Berberine hydrochloride.Conclusion: the characteristic of identification by TLC was distinct and highly specific and the quantification method had the linearity range of 26.64～133.20 μg/mL,r=0.9997.The average recovery was 96.77% and RSD was 1.6%.The methods of identification and quantification are simple,realizable and reproducible.It could be used effectively for the quality control of Shaotangshang gel.

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What this paper is about

Objective: to establish the chromatographic methods of Shaotangshang gel for control the quality standard of Chinese medicine.Methods: TLC was performed to identify Rhizoma coptidis,Cortex phellodendri chinensis,Radix et rhizome rhei,Radix scutellariae.HPLC was used to determine content of Berberine hydrochloride.Conclusion: the characteristic of identification by TLC was distinct and highly specific and the quantification method had the linearity range of 26.64～133.20 μg/mL,r=0.9997.The average recovery was 96.77% and RSD was 1.6%.The methods of identification and quantification are simple,realizable and reproducible.It could be used effectively for the quality control of Shaotangshang gel.

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Available abstract

Objective: to establish the chromatographic methods of Shaotangshang gel for control the quality standard of Chinese medicine.Methods: TLC was performed to identify Rhizoma coptidis,Cortex phellodendri chinensis,Radix et rhizome rhei,Radix scutellariae.HPLC was used to determine content of Berberine hydrochloride.Conclusion: the characteristic of identification by TLC was distinct and highly specific and the quantification method had the linearity range of 26.64～133.20 μg/mL,r=0.9997.The average recovery was 96.77% and RSD was 1.6%.The methods of identification and quantification are simple,realizable and reproducible.It could be used effectively for the quality control of Shaotangshang gel.

Key concepts: Chromatography, Radix (gastropod), Berberine hydrochloride, Chemistry, Quality standard, High-performance liquid chromatography, Biology, Botany

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