ScholarLens Back to search
2008•Di-Si Junyi Daxue xuebaoRequires access

Construction of eukaryotic expression vector of AKTIP gene and its expression in GES-1 cells

Rong Li

Open publisher page 0 citations

Abstract

AIM:To construct eukaryotic expression vector of the gene of AKT interacting protein(AKTIP)and to detect its expression in GES-1 cells.METHODS:Total RNA was extracted from human peripheral blood.The full length cDNA of AKTIP gene was amplified by RT-PCR,and then cloned into pcDNA3.1 /myc-His(-)A vector by KpnI and NheI restrictive enzyme sites.The gene was identified and comfirmed by sequencing,and was then transfected into GES-1 cells,and the expressed AKTIP protein was detected by Western blot analysis.RESULTS:The seqencing result confirmed the eukaryotic expression vector of AKTIP was correctly constructed,and the result of Western blot showed it had a good expression in GES-1 cells.CONCLUSION:pcDNA3.1/myc-His(-)A-AKTIP has been successfully constructed,which can have a good expression in GES-1 cells.This study will provide a foundation for further investigation on the function and molecular constitution of AKTIP.

About this research paper

What this paper is about

AIM:To construct eukaryotic expression vector of the gene of AKT interacting protein(AKTIP)and to detect its expression in GES-1 cells.METHODS:Total RNA was extracted from human peripheral blood.The full length cDNA of AKTIP gene was amplified by RT-PCR,and then cloned into pcDNA3.1 /myc-His(-)A vector by KpnI and NheI restrictive enzyme sites.The gene was identified and comfirmed by sequencing,and was then transfected into GES-1 cells,and the expressed AKTIP protein was detected by Western blot analysis.RESULTS:The seqencing result confirmed the eukaryotic expression vector of AKTIP was correctly constructed,and the result of Western blot showed it had a good expression in GES-1 cells.CONCLUSION:pcDNA3.1/myc-His(-)A-AKTIP has been successfully constructed,which can have a good expression in GES-1 cells.This study will provide a foundation for further investigation on the function and molecular constitution of AKTIP.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

AIM:To construct eukaryotic expression vector of the gene of AKT interacting protein(AKTIP)and to detect its expression in GES-1 cells.METHODS:Total RNA was extracted from human peripheral blood.The full length cDNA of AKTIP gene was amplified by RT-PCR,and then cloned into pcDNA3.1 /myc-His(-)A vector by KpnI and NheI restrictive enzyme sites.The gene was identified and comfirmed by sequencing,and was then transfected into GES-1 cells,and the expressed AKTIP protein was detected by Western blot analysis.RESULTS:The seqencing result confirmed the eukaryotic expression vector of AKTIP was correctly constructed,and the result of Western blot showed it had a good expression in GES-1 cells.CONCLUSION:pcDNA3.1/myc-His(-)A-AKTIP has been successfully constructed,which can have a good expression in GES-1 cells.This study will provide a foundation for further investigation on the function and molecular constitution of AKTIP.

Key concepts: Transfection, Molecular biology, Complementary DNA, Vector (molecular biology), Gene, Biology, Gene expression, Western blot

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Construction of eukaryotic expression vector of AKTIP gene and its expression in GES-1 cells — Research Paper | ScholarLens