ScholarLens Back to search
2003•Di-Si Junyi Daxue xuebaoRequires access

Cloning of sFGFR1 and its high efficient expression in RTS

Yong Chen

Open publisher page 0 citations

Abstract

AIM: To clone sFGFR1 gene and express corresponding protein in RTS. METHODS: Swiss rat 3T3 fibroblasts were cultured, total RNAs were extracted and sFGFR1 cDNA was obtained by RT PCR. The fragments were cut by means of NcoI and SmaI and products were cloned into pIVEX2 3 d vector and sequenced. SFGFR1 protein was expressed by using RTS ProteinMaster 500 and products were analyzed by Western Blot. RESULTS: sFGFR1 gene was cloned and sequencing analysis confirmed the cloned sequence was correct. Western Blot results confirmed sFGFR1 was highly expressed. CONCLUSION: sFGFR1 gene is cloned and highly expressed in RTS.

About this research paper

What this paper is about

AIM: To clone sFGFR1 gene and express corresponding protein in RTS. METHODS: Swiss rat 3T3 fibroblasts were cultured, total RNAs were extracted and sFGFR1 cDNA was obtained by RT PCR. The fragments were cut by means of NcoI and SmaI and products were cloned into pIVEX2 3 d vector and sequenced. SFGFR1 protein was expressed by using RTS ProteinMaster 500 and products were analyzed by Western Blot. RESULTS: sFGFR1 gene was cloned and sequencing analysis confirmed the cloned sequence was correct. Western Blot results confirmed sFGFR1 was highly expressed. CONCLUSION: sFGFR1 gene is cloned and highly expressed in RTS.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

AIM: To clone sFGFR1 gene and express corresponding protein in RTS. METHODS: Swiss rat 3T3 fibroblasts were cultured, total RNAs were extracted and sFGFR1 cDNA was obtained by RT PCR. The fragments were cut by means of NcoI and SmaI and products were cloned into pIVEX2 3 d vector and sequenced. SFGFR1 protein was expressed by using RTS ProteinMaster 500 and products were analyzed by Western Blot. RESULTS: sFGFR1 gene was cloned and sequencing analysis confirmed the cloned sequence was correct. Western Blot results confirmed sFGFR1 was highly expressed. CONCLUSION: sFGFR1 gene is cloned and highly expressed in RTS.

Key concepts: Cloning (programming), Molecular biology, Complementary DNA, clone (Java method), Biology, Gene, Western blot, Sequence analysis

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning of sFGFR1 and its high efficient expression in RTS — Research Paper | ScholarLens