Effects of MicroRNA-99b Modulation on Proliferation and Apoptosis in SiHa Cells

【Objective】 To investigate the effects of microRNA-99b (miR-99b) up-regulation on proliferation and apoptosis in SiHa cells and its possible mechanism. 【Methods】 The SiHa cells were divided into normal control, negative control, and miR-99b modulated groups. Cy3 dye labeled PremiR Negative Control was transfected into negative control cells, and Pre-miR-hsa-miR-99b miRNA Precursor was transfected into miR-99b modulated cells with siPORT NeoFX transfection agent. The miR-99b expression was detected by real-time PCR. The cellular growth activity was assayed by MTT assay, and the apoptosis was tested by flow cytometry. The protein expression of CDC25A was measured by Western blot analysis. 【Results】 The miR-99b expression in miR-99b modulated cells increased 107.23 fold. The cellular growth ratio of normal control group was 3.26% ± 0.44%, of negative control group 3.65% ± 0.47%, of miR-99b modulated group 2.24% ± 0.45%. The apoptosis ratio of normal control group was 8.12% ± 1.54%, of negative control group 8.75% ± 1.67%, of miR-99b modulated group 14.26% ± 1.70%. The CDC25A protein expression of normal control group was 0.50 ± 0.06, of negative control group 0.59 ± 0.05, of miR-99b modulated group 0.31 ± 0.05. The cellular growth ratio, apoptosis ratio, and CDC25A protein expression had significant difference between miR-99b modulated group and two control groups (P 0.05). 【Conclusions】 The miR-99b up-regulation could suppress the cellular proliferation and induce apoptosis in SiHa cells by decreased target genes CDC25A expression. The miR-99b may be a target gene for cervical carcinoma treatment.