2013•Chinese Journal of Clinical Laboratory ScienceRequires access
Simultaneous detection of West Nile virus and Chikungunya virus by duplex real-time quantitative PCR
Yu Beibei, Yi-yu Lu, Xie Xinyou, XU Chang-ping, Jun Zhang
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Abstract
Objective To develop a duplex real-time quantitative PCR assay for simultaneous detection of West Nile virus and Chikungunya virus.Methods Two sets of primers and TaqMan probes were designed based on highly conserved CAP gene region of West Nile virus and E1 gene region of Chikungunya virus,and the reactive condition of duplex real-time PCR was optimized.The sensitivity and specificity of the assay were evaluated.Results The duplex real-time quantitative PCR assay showed excellent specificity in simultaneous detection of West Nile virus and Chikungunya virus.The sensitivities of the assay were 10 copies/μL for both the virus,and the correlation coefficient of the quantitative curve were 0.999 and 0.998 respectively.Conclusion The duplex fluorescent quantitative PCR assay developed in this study is sensitive and specific for simultaneous detection of West Nile virus and Chikungunya virus.The efficiency of the assay for detecting of clinical samples should be further evaluated.