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2008•Zhongguo meijie shengwuxue ji kongzhi zazhiRequires access

Study on quick detection of West Nile virus by TaqMan-based Real-time PCR

Gao Xiao-pin

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Abstract

Objective To establish a TaqMan-based Real-time PCR assay for detection of West Nile virus. Methods The specific primers and probe selected from the references were synthesized in the conserved region of the CAP gene of West Nile virus, and the viral nucleic acid was synthesized by PCR-based gene synthesis method. The PCR condition was optimized to improve the sensitivity and specificity of the assay. Results The specificity of the assay was high and there were no cross reactions with Dengue virus and Japanese encephalitis virus. The sensitivity of the assay was 100 gene copies per cycle. Conclusion This TaqMan-based Real-time PCR assay was a quick, sensitive and specific tool for molecular diagnosis of West Nile virus.

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What this paper is about

Objective To establish a TaqMan-based Real-time PCR assay for detection of West Nile virus. Methods The specific primers and probe selected from the references were synthesized in the conserved region of the CAP gene of West Nile virus, and the viral nucleic acid was synthesized by PCR-based gene synthesis method. The PCR condition was optimized to improve the sensitivity and specificity of the assay. Results The specificity of the assay was high and there were no cross reactions with Dengue virus and Japanese encephalitis virus. The sensitivity of the assay was 100 gene copies per cycle. Conclusion This TaqMan-based Real-time PCR assay was a quick, sensitive and specific tool for molecular diagnosis of West Nile virus.

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Available abstract

Objective To establish a TaqMan-based Real-time PCR assay for detection of West Nile virus. Methods The specific primers and probe selected from the references were synthesized in the conserved region of the CAP gene of West Nile virus, and the viral nucleic acid was synthesized by PCR-based gene synthesis method. The PCR condition was optimized to improve the sensitivity and specificity of the assay. Results The specificity of the assay was high and there were no cross reactions with Dengue virus and Japanese encephalitis virus. The sensitivity of the assay was 100 gene copies per cycle. Conclusion This TaqMan-based Real-time PCR assay was a quick, sensitive and specific tool for molecular diagnosis of West Nile virus.

Key concepts: TaqMan, Virology, Real-time polymerase chain reaction, West Nile virus, Biology, Virus, Dengue virus, Dengue fever

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