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Derermination of emodin and chrysophanol in Shierwei Baixiao capsule by RP-HPLC

Xianghong Chen

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Abstract

OBJECTIVE To establish a method for determination of emodin and chrysophanol in Shierwei Baixiao capsules.METHODS The samples were extracted by supersonic with methanol.RP-HPLC analysis was carried out on Hypersil ODS_2 C_(18) column and the mixture of methanol-0.1%H_3PO_4(85∶15)was used as the mobile phase.The detection wavelength was at 254 nm.RESULTS The standard curve was linear within the range of 1.7-8.4 μg for emodin and 1.6～8.2 μg for chrysophanol.The average recovery of emodin was 99.3% with RSD of 0.8%,with that of chrysophanol was 99.3% with RSD of 0.9%.CONCLUSION The method is sensitive,accurate and specific and can be used for the determination of emodin and chrysopanol in Shierwei Baixiao capsule.

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What this paper is about

OBJECTIVE To establish a method for determination of emodin and chrysophanol in Shierwei Baixiao capsules.METHODS The samples were extracted by supersonic with methanol.RP-HPLC analysis was carried out on Hypersil ODS_2 C_(18) column and the mixture of methanol-0.1%H_3PO_4(85∶15)was used as the mobile phase.The detection wavelength was at 254 nm.RESULTS The standard curve was linear within the range of 1.7-8.4 μg for emodin and 1.6～8.2 μg for chrysophanol.The average recovery of emodin was 99.3% with RSD of 0.8%,with that of chrysophanol was 99.3% with RSD of 0.9%.CONCLUSION The method is sensitive,accurate and specific and can be used for the determination of emodin and chrysopanol in Shierwei Baixiao capsule.

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Available abstract

OBJECTIVE To establish a method for determination of emodin and chrysophanol in Shierwei Baixiao capsules.METHODS The samples were extracted by supersonic with methanol.RP-HPLC analysis was carried out on Hypersil ODS_2 C_(18) column and the mixture of methanol-0.1%H_3PO_4(85∶15)was used as the mobile phase.The detection wavelength was at 254 nm.RESULTS The standard curve was linear within the range of 1.7-8.4 μg for emodin and 1.6～8.2 μg for chrysophanol.The average recovery of emodin was 99.3% with RSD of 0.8%,with that of chrysophanol was 99.3% with RSD of 0.9%.CONCLUSION The method is sensitive,accurate and specific and can be used for the determination of emodin and chrysopanol in Shierwei Baixiao capsule.

Key concepts: Emodin, Chemistry, Chromatography, Capsule, High-performance liquid chromatography, Methanol, Organic chemistry, Botany

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Derermination of emodin and chrysophanol in Shierwei Baixiao capsule by RP-HPLC — Research Paper | ScholarLens