2013•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Rhien,Emodin and Chrysophanol in Weishuanjiaonang by HPLC

Meirong Bai

Abstract

Objective:To establish a method for the determination of rhien,emodin and chrysophanol in Weishuanjiaonang by HPLC.Method:Rhien,emodin and chrysophanol in Weishuanjiaonang was determined by HPLC on VP-ODS(4.6 mm×150 mm,5 μm)column with methyl petchloric acid(0.1%)(85:15)as a mobile phase and the detective wavelength was 254 nm,the flow rate is 0.6 mL·min-1.Result:The good linear relationship of rhien,emodin and chrysophanol in Weishuanjiaonang.The average recovery of rhien was 98.76%,RSD 0.56%;The average recovery of emodin is 97.22%,RSD 0.33%.The average recovery of chrysophanol was 97.53%,RSD 0.76%.Conclusion:The method is simple,rapid and gives satisfying results.It can be suitable for quality control of Weishuanjiaonang.

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Key concepts: Emodin, Chromatography, High-performance liquid chromatography, Chemistry, Linear relationship, Mathematics, Statistics