Effect of propofol pretreatment on the expression of NE following cerebral ischemia-reperfusion injury in rats

Objective: To investigate the effect of propofol pretreatment on the expression of neutropil elastase(NE) following cerebral ischemia-reperfusion(IR) injury in rats.Methods:Thirty healthy male SD rats,weighing 240～280 g,were randomly divided into the control group(Group C),cerebral ischemia-reperfusion group(Group IR) and propofol pretreatment group(Group P,propofol was injected intraperitoneal 10 min before cerebral ischemia)(n=10).Focal cerebral ischemia-reperfusion model was established in rats by reversible middle cerebral artery occlusion with filament.Cerebral ischemia was for 2 hours and then with 24 hours of reperfusion.Neurological deficit score was executed after cerebral ischemia 2 h and reperfusion 24 h in the rats.ELISA assay was used to detect the expression of NE,TNF-α and IL-1β in the brain tissue.Results:Compared with Group C,the activity of NE and the expression of TNF-α and IL-1β in Group IR and Group Propofol were significantly increased(P0.01);Compared with Group IR,the activity of NE and the expression of TNF-α AND IL-1β in Group Propofol were significantly decreased(P0.01).Conclusion:Different dose propofol pretreatment can significantly reduce the activation of NE and further,inhibit the over-expression of TNF-α and IL-1β in the brain tissue following cerebral IR injury,and reduce the inflammatory response,and therefore has a significantly protective effect on cerebral IR injury.