Screening of differentially expressed genes related to dedifferentiated thyroid cancer by cDNA microarray.

Objective To screen the differentially expressed genes between differentiated thyroid cancer and dedifferentiated thyroid cancer tissues using cDNA microarray,and identify new targets and strategies for the pathogenesis of dedifferentiated thyroid cancer. Methods mRNA from both differentiated thyroid cancer and dedifferentiated thyroid cancer tissues were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP(Cy-5 or Cy-3) to prepare the hybridization probes. The mixed probes were hybridized to the cDNA microarray, which was scanned for the fluorescent signals and showed difference between two tissues. Results A series of differentially expressed genes were found between two tissues. 209 up-regulated and 164 down-regulated genes were identified among the 373 differentially expressed genes. Conclusion cDNA microarry provides a promising approach to specific gene expressions of thyroid cancer dedifferentiated.