Effect of Exogenous Abscisic Acid on Tolerance of Wheat Seedlings to Cadmium Stress
Han Chao, Haiyu Shen, Jia Ye, Yang Li, Shuang Liang
Abstract
Han Chao, Haiyu Shen, Jia Ye, Yang Li, Shuang Liang
Abstract
Effects of exogenous abscisic acid(ABA) on the growth and some physio-biochemical signs in wheat(Triticum aestivum L.) seedlings under cadmium stress(100 mg/L) were studied with hydroponics.The results showed that:(1)The malondialdehyde(MDA) content in wheat seedlings was significantly increased(P0.05),and the growth of wheat seedlings was also obviously inhibited by Cd2+ stress.(2)Exogenous ABA with the concentration of 5.0 μmol/L playing a noticeable role increased root activity and proline content,enhanced activities of SOD,POD and CAT,and decreased MDA content.(3)Exogenous 1.0-5.0 μmol/L ABA eased the negative effects of Cd2+ on the growth of wheat seedlings,and 5.0 μmol/L ABA worked best with shoot height,root length and total biomass of seedlings in 5.0 μmol/L ABA and Cd2+ stress had increased 6.73%,149% and 10.52%,respectively,compared with those under single Cd2+ stress.However,10.0 μmol/L ABA exacerbated the growth of wheat seedlings.On the whole,preference concentration of exogenous ABA could ease the inhibitory effects of Cd2+ stress on the growth of wheat seedlings and boost the tolerance of wheat seedlings to Cd2+ stress by increasing SOD,POD and CAT activities and proline content.Furthermore,the most suitable concentration of ABA was defined to be 5.0 μmol/L.
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Effects of exogenous abscisic acid(ABA) on the growth and some physio-biochemical signs in wheat(Triticum aestivum L.) seedlings under cadmium stress(100 mg/L) were studied with hydroponics.The results showed that:(1)The malondialdehyde(MDA) content in wheat seedlings was significantly increased(P0.05),and the growth of wheat seedlings was also obviously inhibited by Cd2+ stress.(2)Exogenous ABA with the concentration of 5.0 μmol/L playing a noticeable role increased root activity and proline content,enhanced activities of SOD,POD and CAT,and decreased MDA content.(3)Exogenous 1.0-5.0 μmol/L ABA eased the negative effects of Cd2+ on the growth of wheat seedlings,and 5.0 μmol/L ABA worked best with shoot height,root length and total biomass of seedlings in 5.0 μmol/L ABA and Cd2+ stress had increased 6.73%,149% and 10.52%,respectively,compared with those under single Cd2+ stress.However,10.0 μmol/L ABA exacerbated the growth of wheat seedlings.On the whole,preference concentration of exogenous ABA could ease the inhibitory effects of Cd2+ stress on the growth of wheat seedlings and boost the tolerance of wheat seedlings to Cd2+ stress by increasing SOD,POD and CAT activities and proline content.Furthermore,the most suitable concentration of ABA was defined to be 5.0 μmol/L.
Key concepts: Abscisic acid, Proline, Malondialdehyde, Hydroponics, Shoot, Point of delivery, Seedling, Cadmium