The Effects of Mifepristone and Gestrinone on Growth and Proliferation in Ectopic and Normal Endometrial Stroma Cells in Vitro

Objective:To investigate mifepristone and gestrinone's effects on growth and proliferation in isolated ectopic and normal endometrial stroma cells in vitro.Methods:Endometrial stroma cells were cultured and exposed to mifepristone or gestrinone of different doses.The inhibition of the cells during 60 hours was determined by methylthiazolyl tetrazolium(MTT) assay,and the cell growth curve was made.At 36 hours the cell cycle and apoptosis rate were monitored by flow cytometry(FCM).Results:Both mifepristone and gestrinone could inhibit the growth and proliferation of endometrial stroma cells in a dose-and time-dependent manner.After 36 hours,the cell cycles were inhibited from G1 stage to S stage,with increased apoptotic rate.Conclusions:① Mifepristone and gestrinone can both significantly inhibit proliferation and induce apoptosis.When compared at the same concentration,the latter has stronger effect.② The inhibition effect of mifepristone and gestrinone to the ectopic endometrial stroma cells was stronger than to those from normal endometrium.