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GENETIC VARIATION OF ISOLATES OF RHIZOCTONIA SOLANI AG-1 IN YUNNAN PROVINCE

Yu Jin

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Abstract

Twelve RAPD primers were used to assess the genetic variation of 13 isolates of Rhizoctonia solani AG-1 from different localities of Yunnan province . A total of 182 polymorphic bands was amplified with 12 primers and no common bands were found among testd isolates. The result showed that the rate of polymorphism of the isolates was 100 percent. The dendrogram derived from RAPD data by Unweighted Pair Group Method using Arithmetic Average (UPGMA) suggested that 13 isolates could be divided into 9 subgroups with the genetic distance of 0.5, 6 subgroups with the genetic distance of 0.86, and 3 subgroups with the genetic distance of 0.95. It was proved that the isolates of AG-1 from different localities of Yunnan manifested abundant genetic diversity.

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What this paper is about

Twelve RAPD primers were used to assess the genetic variation of 13 isolates of Rhizoctonia solani AG-1 from different localities of Yunnan province . A total of 182 polymorphic bands was amplified with 12 primers and no common bands were found among testd isolates. The result showed that the rate of polymorphism of the isolates was 100 percent. The dendrogram derived from RAPD data by Unweighted Pair Group Method using Arithmetic Average (UPGMA) suggested that 13 isolates could be divided into 9 subgroups with the genetic distance of 0.5, 6 subgroups with the genetic distance of 0.86, and 3 subgroups with the genetic distance of 0.95. It was proved that the isolates of AG-1 from different localities of Yunnan manifested abundant genetic diversity.

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Available abstract

Twelve RAPD primers were used to assess the genetic variation of 13 isolates of Rhizoctonia solani AG-1 from different localities of Yunnan province . A total of 182 polymorphic bands was amplified with 12 primers and no common bands were found among testd isolates. The result showed that the rate of polymorphism of the isolates was 100 percent. The dendrogram derived from RAPD data by Unweighted Pair Group Method using Arithmetic Average (UPGMA) suggested that 13 isolates could be divided into 9 subgroups with the genetic distance of 0.5, 6 subgroups with the genetic distance of 0.86, and 3 subgroups with the genetic distance of 0.95. It was proved that the isolates of AG-1 from different localities of Yunnan manifested abundant genetic diversity.

Key concepts: UPGMA, RAPD, Dendrogram, Genetic diversity, Biology, Genetic variation, Rhizoctonia solani, Veterinary medicine

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GENETIC VARIATION OF ISOLATES OF RHIZOCTONIA SOLANI AG-1 IN YUNNAN PROVINCE — Research Paper | ScholarLens