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2010•Medical Journal of West ChinaRequires access

Effect of quercetin on TGF-β stimulated tubular epithelial-myofibroblast transdifferentiation

Ping Wang

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Abstract

Objective To observe the effect of quercetin on the tubular epithelial-myofibroblast transdifferentiation(TEMT) induced by TGF-β in vitro.Methods The cultured NRK52E cells were stimulated by TGF-β and at the same time co-incubated with different concentration of quercetin.After cells been cultured,the expressions of a-SMA,E-cadherin were measured by immunocytochemistry method and PCR.Results TGF-β induced the expression of a-SMA and suppressed the expression of E-cadherin of NRK52E cells.Treated with Quercetin can reduce the TGF-β induced expression of a-SMA,and upregulate the expression of E-cadherin significantly.Conclusion Quercetin can partly inhibit the process of TMET induced by TGF-β.

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What this paper is about

Objective To observe the effect of quercetin on the tubular epithelial-myofibroblast transdifferentiation(TEMT) induced by TGF-β in vitro.Methods The cultured NRK52E cells were stimulated by TGF-β and at the same time co-incubated with different concentration of quercetin.After cells been cultured,the expressions of a-SMA,E-cadherin were measured by immunocytochemistry method and PCR.Results TGF-β induced the expression of a-SMA and suppressed the expression of E-cadherin of NRK52E cells.Treated with Quercetin can reduce the TGF-β induced expression of a-SMA,and upregulate the expression of E-cadherin significantly.Conclusion Quercetin can partly inhibit the process of TMET induced by TGF-β.

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Available abstract

Objective To observe the effect of quercetin on the tubular epithelial-myofibroblast transdifferentiation(TEMT) induced by TGF-β in vitro.Methods The cultured NRK52E cells were stimulated by TGF-β and at the same time co-incubated with different concentration of quercetin.After cells been cultured,the expressions of a-SMA,E-cadherin were measured by immunocytochemistry method and PCR.Results TGF-β induced the expression of a-SMA and suppressed the expression of E-cadherin of NRK52E cells.Treated with Quercetin can reduce the TGF-β induced expression of a-SMA,and upregulate the expression of E-cadherin significantly.Conclusion Quercetin can partly inhibit the process of TMET induced by TGF-β.

Key concepts: Transdifferentiation, Quercetin, Myofibroblast, Transforming growth factor, Medicine, Immunocytochemistry, SMA*, Downregulation and upregulation

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Effect of quercetin on TGF-β stimulated tubular epithelial-myofibroblast transdifferentiation — Research Paper | ScholarLens