Effect of quercetin on epithelial-mesenchymal transition of the human lung epithelial cell line A549 induced by TGF-β

Objective To investigate the effect of Quercetin on epithelial-mesenchymal transition in human lung epithelial cell line A549 induced by TGF-β.Methods A549 cell was divided to 3 groups:no TGF-β group,DMSO control group,and TGF-β(10 ng/mL)+ Quercetin group.After 12,24,48 and 72 h of treatment,proliferation of cell was determined with methabenzthiazuron(MTT) assay.The mRNA expression of E-cadherin,N-cadherin and Vimentin was determined by real-time RT-PCR.The proteins of E-cadherin,N-cadherin and Vimentin were determined by Western blotting.Results Compared with the control group,the proliferation of TGF-β(10 ng/mL)+Quercetin group was significantly decreased.Meanwhile,E-cadherin was increased,N-cadherin and vimentin was obviously up-regulated at mRNA and protein level in A549 cells.Conclusion Quercetin can markedly inhibit the proliferation of lung adenocarcinoma cell line A549 induced by TGF-β in time-dependent and dose-dependent manners,prevent epithelial-interstitial transformation of A549 cells,decrease the migration and metastatic abilities of A549 cells.