Genetic Diversity Analysis of Soybean Germplasm in Heilongjiang Province by ISSR Markers

The genetic diversities of 25 soybean varieties were studied by ISSR markers,9 polymorphic primers were selected from 28 ISSR primers,65 bands were amplified with 9 primers,they contained 58 polymorphic bands and their polymorphism percentage was 89.23%;Shannon's index evaluation indicted that average Shannon's index was 0.4604 and the numbers of effective alleles were 1.5139;the genetic similarity coefficient among the 25 varieties ranged from 0.4462 to 0.8923,and the average GS was 0.6692;cluster analysis with UPGMA method showed that the cultivars tested in this study could be divided into five groups at the level GS 0.698.