PrP106-126 induced neurotoxicity in mouse neuroblastoma cells N2a

To investigate the apoptotic effect of prion peptide PrP106-126 on mouse neuroblastoma cell line,N2a,a flow cytometry assessment with Annexin V-FITC/PI and apoptotic morphology were used to detect apoptosis induced by PrP106-126.The results showed that 15,25 and 50 μmol/L at 24 h substantially altered the morphology and significantly induced apoptosis(P0.05).An increased concentration elevated the apoptotic effect;the 25 μmol/L PrP 106-126 induced cell apoptosis at 24 h and 48 h treatment(P0.05) in a time-dependent manner.However,100 μmol/L at 12 h showed no apoptosis,indicating that the neurotoxicity induced by PrP 106-126 was the chronic result of amyloid-like fibrils.