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2013•Journal of Bengbu Medical CollegeRequires access

Effect of tumor necrosis factor-α on the nitric oxide level in cultured osteoblasts in vitro

H Ji

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Abstract

Objective: To explore the level of nitric oxide( NO) in osteoblasts treated with different concentrations of tumor necrosis factor-α( TNF-α). Methods: The osteoblasts were cultured and identified. The NO level in cultured supernafants in osteoblasts treated with different concentrations of TNF-α was measured. Results: The level of the NO was increased with the increase of concentration of TNF-α,which was the maximum at 10 μg / L of TNF-α. The level of the NO began to decrease with the increase of processing time and concentration of TNF-α( P 0. 05 to P 0. 01). Conclusions: TNF-α can induce the expression of NO,but TNF-α can not countine to increase the expression of NO with the increase of its processing time and concentration in cultured osteoblasts.

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What this paper is about

Objective: To explore the level of nitric oxide( NO) in osteoblasts treated with different concentrations of tumor necrosis factor-α( TNF-α). Methods: The osteoblasts were cultured and identified. The NO level in cultured supernafants in osteoblasts treated with different concentrations of TNF-α was measured. Results: The level of the NO was increased with the increase of concentration of TNF-α,which was the maximum at 10 μg / L of TNF-α. The level of the NO began to decrease with the increase of processing time and concentration of TNF-α( P 0. 05 to P 0. 01). Conclusions: TNF-α can induce the expression of NO,but TNF-α can not countine to increase the expression of NO with the increase of its processing time and concentration in cultured osteoblasts.

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Available abstract

Objective: To explore the level of nitric oxide( NO) in osteoblasts treated with different concentrations of tumor necrosis factor-α( TNF-α). Methods: The osteoblasts were cultured and identified. The NO level in cultured supernafants in osteoblasts treated with different concentrations of TNF-α was measured. Results: The level of the NO was increased with the increase of concentration of TNF-α,which was the maximum at 10 μg / L of TNF-α. The level of the NO began to decrease with the increase of processing time and concentration of TNF-α( P 0. 05 to P 0. 01). Conclusions: TNF-α can induce the expression of NO,but TNF-α can not countine to increase the expression of NO with the increase of its processing time and concentration in cultured osteoblasts.

Key concepts: Tumor necrosis factor alpha, Nitric oxide, Medicine, Tumor necrosis factor α, In vitro, Necrosis, Osteoblast, Endocrinology

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Effect of tumor necrosis factor-α on the nitric oxide level in cultured osteoblasts in vitro — Research Paper | ScholarLens