2009•PubMedRequires access

[Effects of tumor necrosis factor-alpha on the growth of rat osteoblasts].

Liwei Xue, Jun Zhang, Xuxia Wang, Tao Bu, Mei Liu

Abstract

OBJECTIVE: To investigate the effects of tumor necrosis factor alpha (TNF-alpha) on the growth of rat osteoblasts. To find out the mechanisms that TNF-alpha regulates the growth of osteoblasts. METHODS: To assay osteoblasts proliferation by MTT. To assay alkaline phosphatase (ALP) activity of osteoblasts by PP-nitrophenyl phosphate (PNPP). RESULTS: The osteblasts proliferation and the ALP activity decreased in treatment groups, and the significantly lower levels were observed in above 50 ng/mL groups (P<0.05). CONCLUSION: TNF-alpha restrained osteoblasts proliferation and differentiation, and the effects were more significant in above 50 ng/mL groups.

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Key concepts: Alkaline phosphatase, Tumor necrosis factor alpha, Osteoblast, Chemistry, Alpha (finance), Endocrinology, Internal medicine, MTT assay