Research Progress of ChloasmaModel Preparation of Liver Qi Stagnation Pattern/Syndrome in Mice

It is the bottleneck problem to set up the experimental animal model for pattern/syndrome differentiation in accordance with the characteristics of traditional Chinese medicine(TCM) to restrict the pharmacological research in the development of new drug of TCM.Chloasma is the common cosmetic disease.In the research and development of new drug of the disease,the point is to build the model of chloasma differentiated as liver qi stagnation in compliance with the pattern / syndrome differentiation and determination of treatment in TCM.The paper introduced briefly the method of model preparation of chloasma differentiated as liver qi stagnation in mice in recent years and attempted to find the scientific method for the setup of the model of chloasma differentiated as liver qi stagnation in mice so as to provide more systematic and scientific experimental animal model for the clinical research and development of new drug of TCM for the treatment of chloasma.The analysis indicated that progesterone injection + ultraviolet irradiation + bound method was the relatively acceptable approach to the preparation of mice model.The detection of tyrosine and malondialdehyde expressions as well as superoxide dismutases activity in skin tissue could evaluatemuch more completely the changes of biochemical indexes in chloasma.On the basis of the previous research on the animal model of chloasma differentiated as liver qi stagnation,the behavior methods such as open-field test,syrup preference and forced swimming were supplemented,which assessedmore objectively the behavior indexes in liver qi stagnation.